References

Albasan H, Lulich JP, Osborne CA, Lekcharoensuk C, Ulrich LK, Carpenter KA Effects of storage time and temperature on pH, specific gravity, and crystal formation in urine samples from dogs and cats. J Am Vet Med Assoc. 2003; 222:(2)176-179 https://doi.org/10.2460/javma.2003.222.176

Holan KM, Kruger JM, Gibbons SN, Swenson CL Clinical evaluation of a leukocyte esterase test-strip for detection of feline pyuria. Vet Clin Pathol. 1997; 26:(3)126-131 https://doi.org/10.1111/j.1939-165x.1997.tb00723.x

IDEXX. SediVue Dx urine sediment analyser. 2024. https://www.idexx.co.uk/en-gb/veterinary/analyzers/sedivue-dx-analyzer/ (accessed 5 August 2024)

Kovaříková S, Maršálek P, Vrbová K Cystinuria in dogs and cats: what do we know after almost 200 years?. Animals (Basel). 2021; 11:(8) https://doi.org/10.3390/ani11082437

Osborne CA, Stevens JB Urinalysis: a clinical guide to compassionate patient care.: Shawnee Mission: Bayer Corporation; 1999

Reppas G, Foster SF Practical urinalysis in the cat: 1: urine macroscopic examination ‘tips and traps’. J Feline Med Surg. 2016; 18:(3)190-202 https://doi.org/10.1177/1098612X16631228

Urinalysis in companion animals part 2: evaluation of urine chemistry and sediment. 2014. https://todaysveterinarypractice.com/wp-content/uploads/sites/4/2016/06/T1405C09.pdf (accessed 22 July 2024)

Skeldon N, Ristic J Urinalysis, 3rd edn. In: Villiers E, Ristic J (eds). Gloucester: British Small Animal Veterinary Association; 2016

Valenciano AC, DeNicola DB, Cowell RL, Bowles M, Rizzi TE, Tyler R Atlas of canine and feline urinalysis, 1st edn. Hoboken: Wiley-Blackwell Inc; 2017

Yadav SN, Ahmed N, Nath AJ, Mahanta D, Kalita MK Urinalysis in dog and cat: a review. Vet World. 2020; 13:(10)2133-2141 https://doi.org/10.14202/vetworld.2020.2133-2141

Getting the most from urine and sediment analysis

02 October 2024
10 mins read
Volume 29 · Issue 10
Figure 1. Urine dipstick.
Figure 1. Urine dipstick.

Abstract

Urine sediment examination is an integral part of urinalysis which is frequently overlooked as it can be time consuming and requires a functional microscope, a centrifuge and staff with both the time and expertise to perform the exam. Sediment examination allows the operator to identify crystals, casts, cells and bacteria in a urine sample. Failure to perform sediment examination promptly can lead to ageing artefacts which may negatively affect case management. Examination of the urine sediment should ideally be performed within 1–2 hours of urine collection.

Urine sediment analysis can be a time-consuming job, requiring a functional microscope, a centrifuge and staff with both the time and expertise to perform the exam. Ideally, urine samples should be analysed within 1–2 hours of collection to increase confidence in results. A delay in analysis can lead to changes in temperature or pH, which can lead to precipitation or dissolution of crystals, degradation of cells and casts, potential misdiagnosis and inappropriate decisions regarding case management (Valenciano et al, 2017). This article provides practical tips to help veterinarians become more confident in their interpretation of canine and feline urine sediment examination (including crystals, casts and bacteria) and highlight what in-house urinalysis can add to the clinical workup. In-clinic automated analysers, such as the IDEXX SediVue Dx (IDEXX Laboratories UK), can be used in lieu of a standard microscopic examination and can produce urine results using artificial intelligence in around 3 minutes.

Register now to continue reading

Thank you for visiting UK-VET Companion Animal and reading some of our peer-reviewed content for veterinary professionals. To continue reading this article, please register today.